Chemoenzymatic Synthesis of siRNA and sgRNA Using Ligation Technology
Introduction Advancements in RNA therapeutics are increasing the demand for high-quality synthetic oligonucleotides including siRNA (small interfering RNA) and sgRNA (single guide RNA). While conventional solid-phase oligonucleotide synthesis (SPOS) remains the primary method for siRNA and sgRNA production, its limitations in scalability, sustainability, quality, and cost hinder its ability to meet the anticipated market demands….
